Fractionation of DNA on Hydroxyapatite with a Base-Specific Complexing Agent

Fractionation of DNA on hydroxyapatite with a base-specific complexing agent.

We describe a chromatographic technique for separating mixtures of DNA of varying G + C content. The method employs a specially prepared high-capacity hydroxyapatite and a G.C-specific DNA ligand (2-methyl-3-amino-7-dimethylamino-5-phenyl-phenazinium cation, abbreviated PNR). DNA molecules rich in G.C pairs bind larger amounts of this dye and are eluted earlier from the hydroxyapatite column th...

Base specific fractionation of double stranded DNA: affinity chromatography on a novel type of adsorbant.

A material suitable for base-pair-specific "affinity chromatography" of double stranded DNA is described. The synthesis of this material involves two successive polymerization reactions yielding solid particles of cross-linked bisacrylamide to which base-pair-specific dyes are covalently attached by spacers of polyacrylamide chains of different length. Materials with immobilized A.T-specific ma...

Effect of chelating agent on Hydroxyapatite morphology under hydrothermal process

Citrate was used as chelating or morphology regulating agent in this study. In the presence of regulating agent a two-dimensional monetite phase evolved into  three-dimentional hydroxyapatite microspheres with an average diameter of 4 µm. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) studies revealed the importance of Ca/citrate ratio in regulating the particle m...

Competitive Spectrophotometric Study of Copper, Zinc and Cadmium With 18C6 Using TAR as a Colorimetric Complexing Agent in Methanol

A Competitive spectrophotometric method has been used for the study of complexation between Cu2+, Zn2+ and Cd2+ (M) and 18-crown-6(18C6, B) in methanol using 4-(2-thiazolylazo) resorcinol (TAR, HL) as the colorimetric complexant. Absorption spectra of the species in the M-TAR-18 C6 system at a various concentrations of 18C6 showed no formation of ternary com...

Tracking an ion complexing agent within bilayers